Review



β gal sensor probe  (Biosynth Carbosynth)


Bioz Verified Symbol Biosynth Carbosynth is a verified supplier
Bioz Manufacturer Symbol Biosynth Carbosynth manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Biosynth Carbosynth β gal sensor probe
    Comparison of the 14-day vs 7-day lacZ <t>/β-gal</t> <t>SENSOR</t> probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.
    β Gal Sensor Probe, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+gal+sensor+probe/AquaSpark+beta-D-galactoside%2C+10+mM+in+DMSO%2C+Ramot+at+Tel-Aviv+University+Ltd%2E+Patent+family+WO+2017%2F130191/pmc12910577-87-16-21
    Average 94 stars, based on 1 article reviews
    β gal sensor probe - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Using Next Generation Chemiluminescent Probes to Improve the Plasmodium falciparum in vitro Parasite Reduction Ratio (PRR) Assay"

    Article Title: Using Next Generation Chemiluminescent Probes to Improve the Plasmodium falciparum in vitro Parasite Reduction Ratio (PRR) Assay

    Journal: ACS Infectious Diseases

    doi: 10.1021/acsinfecdis.5c00924

    Comparison of the 14-day vs 7-day lacZ /β-gal SENSOR probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.
    Figure Legend Snippet: Comparison of the 14-day vs 7-day lacZ /β-gal SENSOR probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.

    Techniques Used: Comparison

    Related Articles

    Concentration Assay:

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: .. The β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was diluted to 4 μM in H 2 O containing 1 mM MgCl 2 and added to all wells of the plate to a final concentration of 2 μM (where indicated, 10 μM end concentration was used). .. Plates were incubated for 30–60 min at 37 °C, and the luminescence was quantified using the multimode reader Spark from TECAN at 37 °C.

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Negative Control:

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Incubation:

    Article Title: Next Generation Chemiluminescent Probes for Antimalarial Drug Discovery
    Article Snippet: Luminescence stability was quantified using a theoretical parasite per well range of 20 000–1, 3-fold diluted, and lysed in H 2 O (20 μL per well of a 384-well plate Greiner Bio-One, cat. #781086). .. 20 000 F12 wt parasites were used as negative control. β-gal SENSOR probe (Biosynth AG, cat. #A-8169_P00) was dissolved in PBS containing 1 mM MgCl 2 to a concentration of 20 μM, incubated at RT for 30–60 min and subsequently added to each well to a final concentration of 10 μM. .. Chemiluminescence was measured every 15 min (0–180 min) using PerkinElmer’s IVIS Spectrum In Vivo Imaging System Lumina LT Series III at RT.

    Purification:

    Article Title: Using Next Generation Chemiluminescent Probes to Improve the Plasmodium falciparum in vitro Parasite Reduction Ratio (PRR) Assay
    Article Snippet: .. All wells of the 7- and 14-day lacZ /β-gal SENSOR probe PRR assay were treated with β-gal SENSOR probe (AquaSpark beta-D-galactoside, Biosynth AG, cat. #A-8169_P00) diluted in purified, sterile-filtered water containing MgCl 2 to achieve final concentrations of 10 μM β-gal SENSOR probe and 200 μM MgCl 2 per well. .. The plates were then incubated at 37 °C for 30–60 min. Luminescence signals (counts/s) were measured using the multimode reader Spark from TECAN at 37 °C (exposure time 5 s).

    Sterility:

    Article Title: Using Next Generation Chemiluminescent Probes to Improve the Plasmodium falciparum in vitro Parasite Reduction Ratio (PRR) Assay
    Article Snippet: .. All wells of the 7- and 14-day lacZ /β-gal SENSOR probe PRR assay were treated with β-gal SENSOR probe (AquaSpark beta-D-galactoside, Biosynth AG, cat. #A-8169_P00) diluted in purified, sterile-filtered water containing MgCl 2 to achieve final concentrations of 10 μM β-gal SENSOR probe and 200 μM MgCl 2 per well. .. The plates were then incubated at 37 °C for 30–60 min. Luminescence signals (counts/s) were measured using the multimode reader Spark from TECAN at 37 °C (exposure time 5 s).



    Similar Products

    β gal sensor probe  (Biosynth Carbosynth)
    94
    Biosynth Carbosynth β gal sensor probe
    Comparison of the 14-day vs 7-day lacZ <t>/β-gal</t> <t>SENSOR</t> probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.
    β Gal Sensor Probe, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+gal+sensor+probe/AquaSpark+beta-D-galactoside%2C+10+mM+in+DMSO%2C+Ramot+at+Tel-Aviv+University+Ltd%2E+Patent+family+WO+2017%2F130191/pmc12910577-87-16-21
    Average 94 stars, based on 1 article reviews
    β gal sensor probe - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of the 14-day vs 7-day lacZ /β-gal SENSOR probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.

    Journal: ACS Infectious Diseases

    Article Title: Using Next Generation Chemiluminescent Probes to Improve the Plasmodium falciparum in vitro Parasite Reduction Ratio (PRR) Assay

    doi: 10.1021/acsinfecdis.5c00924

    Figure Lengend Snippet: Comparison of the 14-day vs 7-day lacZ /β-gal SENSOR probe PRR assay in comparison to the published PRR v2 assay. The four reference drugs artemisinin (58 nM), atovaquone (100 nM), chloroquine (112 nM) and pyrimethamine (170 nM) were tested in at least three independent experiments. Time-killing profiles were normalized with error bars representing the standard error of the mean (SEM) of the independent experiments in technical quadruplicates.

    Article Snippet: All wells of the 7- and 14-day lacZ /β-gal SENSOR probe PRR assay were treated with β-gal SENSOR probe (AquaSpark beta-D-galactoside, Biosynth AG, cat. #A-8169_P00) diluted in purified, sterile-filtered water containing MgCl 2 to achieve final concentrations of 10 μM β-gal SENSOR probe and 200 μM MgCl 2 per well.

    Techniques: Comparison